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CNB is a CaM subfamily member which associates with calcineurin A to form the protein phosphatase calcineurin. Shown here is the Ca2+-bound form of CNB. The structure of Ca2+-free CNB has not been solved, but one may postulate that given the similar positioning of the Ca2+-bound EF-hands to those of Ca2+-CaM and Ca2+-TnC (open conformation; compare with the parameters below), the apo EF-hands of CnB may display the closed conformation. EF1 of CNB is shortened in the diagram due to missing residues in the PDB file. |
| EF-hand (helix residues) | N-terminal coordinate
of second helix |
theta | phi | omega |
| 1 (22-29, 39-43) - shortened | (8.490, -0.445, -7.418) | 84.055 | 101.981 | N/A |
| 2 (54-61, 71-81) | (8.283, -0.579, -6.226) | 67.749 | 96.522 | 100 |
| 3 (91-98, 108-118) | (5.301, -1.420, -9.091) | 85.032 | 104.909 | 83 |
| 4 (132-139, 149-159) | (9.824, 0.860, -5.053) | 80.391 | 85.209 | 122 |
NB: values obtained using apo-CaM EF1 (as in refcam.pdb) as the reference EF-hand.