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UNDETERMINED OR DISPUTED CALMODULIN BINDING DOMAINS


This section lists sequences that have been shown to bind calmodulin (typically using peptides), but are not known to adhere to a particular known motif.  In addition, not enough information is available to further search these entries, for one of the following reasons:
  • The reported CaM binding domain is too large (as usually determined by truncation mutants, the sequence range known to bind CaM exceeds the typical 30 residues).
  • There is dispute in the literature regarding CaM binding to the reported region of the target protein.
  • Binding was demonstrated, but a binding site was not identified.   (We have tried our best to include include all CaM targets reported in PubMed; however, please e-mail us if you know of a CaM binding protein not listed here or elsewhere on this site.)

Protein

Reference PMID

Arc35p (yeast homologue of subunit 2 of Arp2/3 complex) 10749918, 10639338
Iqg1p (yeast homologue of IQGAP) 9679143
Gad1p (yeast homologue of glutamate decarboxylase, GAD) 11031268
Dst1p/Ppr2p (yeast homologue of TFIIS) 7925277
NumA (nucleomorphin, Dictyostelium; aa 172-185, 182-194?) 11919178
Rab3B 11741295
p85 11944929, 11942614
syntaxin 13 11329382
CRAG (aa 655-824) 9813038
calossin 9813038
MP20, MP26 (lens fiber cell membrane protein) 8529419, 11335105
NF-kappaB 11752219
EDF-1 (endothelial differentiation-related factor 1) 10816571
tetrahymena EF-1 alpha 10731666
p95PKL 10330411
Estrogen receptor 11278648
Ryanodine receptor 1 (RyR1, skeletal muscle calcium release channel) site 4 4302-4430 (apo) (sites 1,3 in 'other' subclass, site 2 in 1-14 subclass) 8049209, 11306590
Actomyosin 11259447
casein kinase 2 (CK2) catalytic subunit alpha 11827172
myelin basic protein 1693693
PFR-A, -C (paraflagellar rod complex subunits) 10960180
p30, p32 (bull spermatozoa proteins) 10819795
CaMBPs involved in c-Fos expression in medial preoptic area 11398183
myosin, rat (myr4) -2 somewhere in aa:776-874 (site 1 in IQ subclass) 8034741
pituary adenylyl cyclase activating polypeptide (PACAP)-38 7887941
resistance-like gene (RLG) TIR domain fused to soybean CaM-binding domain 12524363
epidermal growth factor receptor (EGFR) aa 644-688 12359306
alpha-synuclein  - N terminal region 12358748
OsCBK, rice CaM binding protein kinase within truncations 1-455 and 408-597 12160464
Cabin1 (Cain) (aa ~2169-2190 according to alignment in PMID 10825153) 10531067
HDAC4 (histone deacetylase 4) (aa ~172-193) 10825153
HDAC5 (histone deacetylase 5) (aa ~184-205) 12626519
Smad1 -1 (aa 1-50; add'l site 2 aa 86-95, Others subclass) 11007779
Smad2 -1 (aa 1-50; add'l site 2 aa 126-135, Others subclass) 11007779
Arabidopsis catalase 3 AtCat3 aa 415-451 11891305
N-type Ca2+ channel (alpha 1B) KVYAALMIFDFYKQNKTSRDQ labelled IQ
starting at I (no Q!) but looks like 1-5-10 from V or Y
10197534,11005820
R-type Ca2+ channel (alpha 1E) KIYAAMMIMDYYKQSKVKKQR labelled IQ
starting at second I (no Q) but looks like 1-5-10 from I or Y
10197534,11005820
P/Q-type Ca2+ channel (alpha 1A) -2 KIYAAMMIMEYYRQSKAKKLQ labelled IQ starting at second I (no Q) but looks like 1-5-10 from I or Y (add'l site 1 in Others subclass) 11005820,11373682
short transient receptor potential channel 2 (Trp2, TrpC2) -1 aa 104-195 (109-161?) (add'l site 2 in Others subclass) 12601176
myosin Ia (brush border), chicken -2 QILISAWFRGHMQRNRYKQM identified as IQ starting at second I (no Q) but might be 1-14 (P47807:727) (add'l sites 1,3 in IQ subclass, sites 4-6 in IQ-like subclass) 9882432
dystrophin -3 aa 3293-3349 but implied 3318-3349 in PMID 9063877 (no proof?) (add'l sites 1,2 in Others subclass) 8636075
utrophin -1 aa 34-58 by homology to dystrophin -1 (see Others subclass) 9063877
utrophin -2 aa 120-141 by homology to dystrophin -2 (see Others subclass) 9063877
utrophin -3 aa 3045-3125 10767429
Arabidposis DRL1 (deformed roots and leaves 1) - last 100 residues bind, possibly aa 257-273 12615938
phospholipase A2 beta -1 (add'l site 2 in Others subclass) aa 615-640 HEYNQDMIRKGQGNKVKKLSIVVSLG protected from trypsinolysis 11118454
adenylyl cyclase, anthrax (edema factor) solved structure (PMID 11807546) shows extensive CaM contacts with the target that are not localized to short sequences 11807546,12724328
RalB: C-terminus of RalA and RalB CaM binding domain is Ca2+-dependent, N-terminus CaM binding domain is Ca2+-independent 12034722
Trk, tyrosine kinase receptors for neurotrophins, aa 669-790 14690530
CP309, homologous to kendrin and CG-NAP but dissimilar CaM binding domain 14565985
Fas (APO-1/CD95) death domain, aa 230-270 containing possible 1-5-10 VKGFVRKNGV 14594800
ErbB2, Neu, HER2 15080792